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Image Search Results
Journal: Frontiers in Microbiology
Article Title: Survey and Visual Detection of Zaire ebolavirus in Clinical Samples Targeting the Nucleoprotein Gene in Sierra Leone
doi: 10.3389/fmicb.2015.01332
Figure Lengend Snippet: The most appropriate primers and reaction temperatures for the reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay. Turbidity was monitored and recorded every 6 s for five sets of primers used to amplify the target gene with a Loopamp real-time turbidimeter at 650 nm. (A) A total of five sets of primers including EBL-1, EBL-2, EBL-7, EBL-11, and EBL-16 were designed to detect artificial EBOV RNA. (B) Reaction temperatures ranged from 53 to 67°C with 2°C intervals.
Article Snippet: To illustrate RT-LAMP detection sensitivity, we targeted a region of NP using the
Techniques: Reverse Transcription, Amplification, RT Lamp Assay
Journal: Frontiers in Microbiology
Article Title: Survey and Visual Detection of Zaire ebolavirus in Clinical Samples Targeting the Nucleoprotein Gene in Sierra Leone
doi: 10.3389/fmicb.2015.01332
Figure Lengend Snippet: Specificity of EBOV NP detection by RT-LAMP. (A) Turbidity was monitored and recorded every 6 s by a Loopamp real-time turbidimeter at 650 nm. (B) Visual detection using a calcein fluorescent detection reagent. Lane 1, positive control (artificial EBOV RNA); lane 2, negative control (double-distilled water); lane 3, Sudan EBOV (artificial Sudan EBOV RNA); lane 4, MARV (artificial MARV RNA); lane 5, SARS coronavirus; lane 6, H7N9; lane 7, H1N1; lane 8, H2N3; lanes 9–12, human parainfluenza viruses (PIV) 1, 2, 3, and 4; lanes 13–15, adenoviruses (ADV; serotypes 3, 5, and 55); lanes 16 and 17, respiratory syncytial virus infection, RSVA, RSVB; lane 18, MERS RNA; lane 19, human metapneumovirus, HMPV; lane 20, bocavirus, BoV; lanes 21–24, human coronavirus, HCoV-229E, HCoV-OC43, HCoV-NL63, and HCoV-HKU1; lane 25, Legionella pneumophila 9135; lane 26, Mycobacterium tuberculosis 005; and lane 27, Haemophilus influenza ATCC 49247.
Article Snippet: To illustrate RT-LAMP detection sensitivity, we targeted a region of NP using the
Techniques: Positive Control, Negative Control, Virus, Infection
Journal: Frontiers in Microbiology
Article Title: Survey and Visual Detection of Zaire ebolavirus in Clinical Samples Targeting the Nucleoprotein Gene in Sierra Leone
doi: 10.3389/fmicb.2015.01332
Figure Lengend Snippet: Comparison of RT-LAMP sensitivities in detecting EBOV NP . Artificial EBOV RNA was serially diluted 10-fold from 4.56 × 10 4 copies/μL to 4.56 × 10 -2 copies/μL. (A) Turbidity was monitored with a Loopamp Realtime Turbidimeter at 650 nm every 6 s. (B) The reaction was detected visually using a calcein fluorescent detection reagent. Artificial EBOV RNA concentrations were: tube 1, 4.56 × 10 4 copies/μL; tube 2, 4.56 × 10 3 copies/μL; tube 3, 4.56 × 10 2 copies/μL; tube 4, 4.56 × 10 1 copies/μL; tube 5, 4.56 copies/μL; tube 6, 4.56 × 10 -1 copies/μL; tube 7, 4.56 × 10 -2 copies/μL; tube 8, ddH 2 O.
Article Snippet: To illustrate RT-LAMP detection sensitivity, we targeted a region of NP using the
Techniques: Comparison
Journal: Frontiers in Microbiology
Article Title: Survey and Visual Detection of Zaire ebolavirus in Clinical Samples Targeting the Nucleoprotein Gene in Sierra Leone
doi: 10.3389/fmicb.2015.01332
Figure Lengend Snippet: Visual detection of RT-LAMP findings of 413 patients with clinically suspected infections in Sierra Leone. Sample information is listed in Supplemental Tables and . A total of 307 positive (A) and 106 negative (B) samples were tested for EBOV infection by real-time RT-PCR and RT-LAMP. NC, negative control (distilled water); PC, positive control (artificial EBOV RNA).
Article Snippet: To illustrate RT-LAMP detection sensitivity, we targeted a region of NP using the
Techniques: Infection, Quantitative RT-PCR, Negative Control, Positive Control